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TNF alpha ELISA Kits

ELISA kits are commonly used to measure soluble biomarkers across a variety of research areas. ELISA kits for TNF alpha are available for Human, Mouse, Rat, Porcine and Baboon which can be quantified in various samples, including serum, supernatant, plasma, tissue homogenate, urine.... ELISA kits are commonly used to measure soluble biomarkers across a variety of research areas. ELISA kits for TNF alpha are available for Human, Mouse, Rat, Porcine and Baboon which can be quantified in various samples, including serum, supernatant, plasma, tissue homogenate, urine.

Invitrogen ELISA kits exist in two...
ELISA kits are commonly used to measure soluble biomarkers across a variety of research areas. ELISA kits for TNF alpha are available for Human, Mouse, Rat, Porcine and Baboon which can be quantified in various samples, including serum, supernatant, plasma, tissue homogenate, urine.

Invitrogen ELISA kits exist in two formats: Uncoated and Coated. Uncoated ELISA kits include all the necessary reagents to coat your own plates and run your assay with maximum flexibility. Coated ELISA kits...
ELISA kits are commonly used to measure soluble biomarkers across a variety of research areas. ELISA kits for TNF alpha are available for Human, Mouse, Rat, Porcine and Baboon which can be quantified in various samples, including serum, supernatant, plasma, tissue homogenate, urine.

Invitrogen ELISA kits exist in two formats: Uncoated and Coated. Uncoated ELISA kits include all the necessary reagents to coat your own plates and run your assay with maximum flexibility. Coated ELISA kits are ready-to-use and quality tested for sensitivity, specificity, precision and lot-to-lot consistency.

TNF alpha detection is also available using highly-sensitive ProQuantum Immunoassays. These assays provide a fast, easy workflow with no wash steps, and are detectable with any real-time PCR instrument.

Can't find what you're looking for? Try the ELISA Builder to design your own assay based on target type and experimental needs. We also offer custom ELISA development services.

Target Information

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

Synonyms

APC1 protein; Cachectin; C-domain 1; C-domain 2; cTNF; DADB-70P7.1; DIF; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; RATTNF; Tnf; TNF alpha; TNF superfamily; TNF α; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNF-a; TNFalpha; TNF-alpha; Tnfsf1a; TNFSF2; TNFα; TNLG1F; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor alpha (cachetin); tumor necrosis factor alpha precursor; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha; tumor necrosis factor-alpha precursor; tumor-necrosis factor; tumour necrosis factor

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Invitrogen
Human TNF alpha Uncoated ELISA Kit
Invitrogen
Human TNF alpha Uncoated ELISA Kit

16 References

Sensitivity 4 pg/mL
Assay Range 4-500 pg/mL
Sample Volume
Plasma
100 µL
Serum
100 µL
Supernatant
100 µL
Time To Result
24 hr 30 min
(1 hr 45 min hands-on)
Price
Special offer
Online exclusive
Online offer:
Cat # 88-7346-88
Invitrogen
Mouse TNF alpha ELISA Kit
Invitrogen
Mouse TNF alpha ELISA Kit

24 References

Sensitivity 3.7 pg/mL
Assay Range 31.3-2,000 pg/mL
Sample Volume
Plasma
50 µL
Serum
50 µL
Supernatant
50 µL
Time To Result
3 hr 30 min
(1 hr 20 min hands-on)
Price
Special offer
Online exclusive
Online offer:
Cat # BMS607-3
Invitrogen
Mouse TNF alpha Uncoated ELISA Kit
Invitrogen
Mouse TNF alpha Uncoated ELISA Kit

46 References

Sensitivity 8 pg/mL
Assay Range 8-1,000 pg/mL
Sample Volume
Lavage Fluid
100 µL
Plasma
100 µL
Serum
100 µL
Time To Result
24 hr 30 min
(1 hr 45 min hands-on)
Price
Special offer
Online exclusive
Online offer:
Cat # 88-7324-88
Invitrogen
Human TNF alpha ProQuantum Immunoassay Kit
Invitrogen
Human TNF alpha ProQuantum Immunoassay Kit

4 References

Sensitivity <0.01 pg/mL
Assay Range 0.0128-5,000 pg/mL
Sample Volume
Serum
2-5 µL
Supernatant
2-5 µL
Plasma
2-5 µL
Time To Result
2 hr
(30 min hands-on)
Price
Special offer
Online exclusive
Online offer:
Cat # A35601
Advanced qPCR technology that provides superior sensitivity, speed, and smaller sample volume. Learn more about ProQuantum™
Invitrogen
Human TNF alpha (Total) ELISA Kit
Invitrogen
Human TNF alpha (Total) ELISA Kit

4 References

Sensitivity 5.0 pg/mL
Assay Range 23-1,500 pg/mL
Sample Volume
Plasma
50 µL
Serum
50 µL
Supernatant
50 µL
Time To Result
3 hr 10 min
(1 hr 20 min hands-on)
Price
Special offer
Online exclusive
Online offer:
Cat # BMS2034
Invitrogen
Human TNF alpha ELISA Kit
Invitrogen
Human TNF alpha ELISA Kit

2 References

Sensitivity 2.3 pg/mL
Assay Range 7.8-500 pg/mL
Sample Volume
Plasma
50 µL
Serum
50 µL
Supernatant
50 µL
Time To Result
3 hr 10 min
(1 hr 20 min hands-on)
Price
Special offer
Online exclusive
Online offer:
Cat # BMS223-4
Invitrogen
Human TNF alpha ELISA Kit
Invitrogen
Human TNF alpha ELISA Kit

32 References

Sensitivity 3 pg/mL
Assay Range 15-1,500 pg/mL
Sample Volume
Plasma
200 µL
Serum
200 µL
Supernatant
200 µL
Time To Result
4 hr 30 min
(1 hr 20 min hands-on)
Price
Special offer
Online exclusive
Online offer:
Cat # KAC1751

96 Tests

Invitrogen
Human TNF alpha ELISA Kit
Invitrogen
Human TNF alpha ELISA Kit

94 References

Sensitivity 1.7 pg/mL
Assay Range 15.6-1,000 pg/mL
Sample Volume
Plasma
100 µL
Serum
100 µL
Supernatant
100 µL
Time To Result
4 hr
(1 hr 20 min hands-on)
Price
Special offer
Online exclusive
Online offer:
Cat # KHC3011
Invitrogen
Human TNF alpha Instant ELISA™ Kit
Invitrogen
Human TNF alpha Instant ELISA™ Kit

3 References

Sensitivity 1.65 pg/mL
Assay Range 7.8-500 pg/mL
Sample Volume
Amniotic Fluid
50 µL
Plasma
50 µL
Serum
50 µL
Time To Result
3 hr 10 min
(15 min hands-on)
Price
Special offer
Online exclusive
Online offer:
Cat # BMS223INST

128 Tests

Invitrogen
Human TNF alpha ELISA Kit, Ultrasensitive
Invitrogen
Human TNF alpha ELISA Kit, Ultrasensitive

28 References

Sensitivity <0.09 pg/mL
Assay Range 0.5-32 pg/mL
Sample Volume
Plasma
50 µL
Serum
50 µL
Supernatant
50 µL
Time To Result
3 hr
(1 hr 20 min hands-on)
Price
Special offer
Online exclusive
Online offer:
Cat # KHC3014
Invitrogen
Human TNF alpha ELISA Kit, High Sensitivity
Invitrogen
Human TNF alpha ELISA Kit, High Sensitivity
Sensitivity 0.13 pg/mL
Assay Range 0.31-20.0 pg/mL
Sample Volume
Serum
50 µL
Plasma
50 µL
Supernatant
50 µL
Time To Result
4 hr
(1 hr 20 min hands-on)
Price
Special offer
Online exclusive
Online offer:
Cat # BMS223-2HS

96 Tests

Invitrogen
Human TNF alpha Uncoated ELISA Kit with Plates
Invitrogen
Human TNF alpha Uncoated ELISA Kit with Plates

41 References

Sensitivity 4 pg/mL
Assay Range 4-500 pg/mL
Sample Volume
Plasma
100 µL
Serum
100 µL
Supernatant
100 µL
Time To Result
24 hr 30 min
(1 hr 45 min hands-on)
Price
Special offer
Online exclusive
Online offer:
Cat # 88-7346-22
Invitrogen
Human TNF alpha ELISA Development Kit (TMB), PeproTech®
Invitrogen
Human TNF alpha ELISA Development Kit (TMB), PeproTech®
Sensitivity Lot-specific
Assay Range Lot-specific
Sample Volume
Time To Result
24 hr 30 min
(1 hr 45 min hands-on)
Price
Special offer
Online exclusive
Online offer:
Cat # 900-T25K

10 x 96 Tests

Invitrogen
Human TNF alpha ELISA Development Kit (ABTS), PeproTech®
Invitrogen
Human TNF alpha ELISA Development Kit (ABTS), PeproTech®
Sensitivity Lot-specific
Assay Range Lot-specific
Sample Volume
Time To Result
24 hr 30 min
(1 hr 45 min hands-on)
Price
Special offer
Online exclusive
Online offer:
Cat # 900-K25K

10 x 96 Tests

Invitrogen
Human TNF alpha Matched Antibody Pair
Invitrogen
Human TNF alpha Matched Antibody Pair
Sensitivity 23.4 pg/mL
Assay Range 23.4-1,500 pg/mL
Sample Volume
Plasma
50 µL
Serum
50 µL
Supernatant
50 µL
Time To Result
24 hr
(1 hr 10 min hands-on)
Price
Special offer
Online exclusive
Online offer:
Cat # BMS2034MST

10 x 96 Tests

Invitrogen
Human TNF alpha Matched Antibody Pair
Invitrogen
Human TNF alpha Matched Antibody Pair

16 References

Sensitivity <15.6 pg/mL
Assay Range 15.6-1,000 pg/mL
Sample Volume
Plasma
100 µL
Serum
100 µL
Supernatant
100 µL
Time To Result
24 hr
(1 hr 30 min hands-on)
Price
Special offer
Online exclusive
Online offer:
Cat # CHC1753

10 x 96 Tests

Invitrogen
Mouse TNF alpha ProQuantum Immunoassay Kit
Invitrogen
Mouse TNF alpha ProQuantum Immunoassay Kit

1 Reference

Sensitivity <0.3 pg/mL
Assay Range 0.32-5,000 pg/mL
Sample Volume
Serum
2-5 µL
Plasma
2-5 µL
Supernatant
2-5 µL
Time To Result
2 hr
(30 min hands-on)
Price
Special offer
Online exclusive
Online offer:
Cat # A43658
Advanced qPCR technology that provides superior sensitivity, speed, and smaller sample volume. Learn more about ProQuantum™
Invitrogen
Mouse TNF alpha Instant ELISA™ Kit
Invitrogen
Mouse TNF alpha Instant ELISA™ Kit
Sensitivity 4.0 pg/mL
Assay Range 31.3-2,000 pg/mL
Sample Volume
Plasma
50 µL
Serum
50 µL
Supernatant
50 µL
Time To Result
3 hr 10 min
(15 min hands-on)
Price
Special offer
Online exclusive
Online offer:
Cat # BMS607-2INST

128 Tests

Invitrogen
Mouse TNF alpha ELISA Kit, High Sensitivity
Invitrogen
Mouse TNF alpha ELISA Kit, High Sensitivity
Sensitivity 0.75 pg/mL
Assay Range 3.13-200 pg/ml
Sample Volume
Serum
50 µL
Plasma
50 µL
Supernatant
50 µL
Time To Result
4 hr
(1 hr 20 min hands-on)
Price
Special offer
Online exclusive
Online offer:
Cat # BMS607-2HS

96 Tests

Invitrogen
Mouse TNF alpha Uncoated ELISA Kit with Plates
Invitrogen
Mouse TNF alpha Uncoated ELISA Kit with Plates

114 References

Sensitivity 8 pg/mL
Assay Range 8-1,000 pg/mL
Sample Volume
Lavage Fluid
100 µL
Plasma
100 µL
Serum
100 µL
Time To Result
24 hr 30 min
(1 hr 45 min hands-on)
Price
Special offer
Online exclusive
Online offer:
Cat # 88-7324-22
Invitrogen
Mouse TNF alpha ELISA Development Kit (ABTS), PeproTech®
Invitrogen
Mouse TNF alpha ELISA Development Kit (ABTS), PeproTech®
Sensitivity Lot-specific
Assay Range Lot-specific
Sample Volume
Time To Result
24 hr 30 min
(1 hr 45 min hands-on)
Price
Special offer
Online exclusive
Online offer:
Cat # 900-K54K

10 x 96 Tests

Invitrogen
Mouse TNF alpha ELISA Development Kit (TMB), PeproTech®
Invitrogen
Mouse TNF alpha ELISA Development Kit (TMB), PeproTech®
Sensitivity Lot-specific
Assay Range Lot-specific
Sample Volume
Time To Result
24 hr 30 min
(1 hr 45 min hands-on)
Price
Special offer
Online exclusive
Online offer:
Cat # 900-T54K

10 x 96 Tests

Invitrogen
Rat TNF alpha ELISA Kit
Invitrogen
Rat TNF alpha ELISA Kit

32 References

Sensitivity 11.0 pg/mL
Assay Range 39.1-2,500 pg/mL
Sample Volume
Serum
50 µL
Supernatant
50 µL
Time To Result
3 hr 10 min
(1 hr 20 min hands-on)
Price
Special offer
Online exclusive
Online offer:
Cat # BMS622
Invitrogen
Rat TNF alpha ELISA Kit
Invitrogen
Rat TNF alpha ELISA Kit

96 References

Sensitivity <4 pg/mL
Assay Range 11.7-750 pg/mL
Sample Volume
Plasma
50 µL
Serum
50 µL
Supernatant
50 µL
Time To Result
4 hr
(1 hr 20 min hands-on)
Price
Special offer
Online exclusive
Online offer:
Cat # KRC3011
Invitrogen
Rat TNF-alpha ELISA Kit
Invitrogen
Rat TNF-alpha ELISA Kit
Sensitivity 25 pg/mL
Assay Range 25-20,000 pg/mL
Sample Volume
Supernatant
50 µL
Plasma
50 µL
Serum
50 µL
Time To Result
4 hr 45 min
(1 hr 20 min hands-on)
Price
Special offer
Online exclusive
Online offer:
Cat # ERA56RB
Invitrogen
Rat TNF-alpha ELISA (For Lysates) Kit
Invitrogen
Rat TNF-alpha ELISA (For Lysates) Kit
Sensitivity 25 pg/mL
Assay Range 25-20,000 pg/mL
Sample Volume
Cell Lysate
50 µL
Tissue Homogenate
50 µL
Time To Result
4 hr 45 min
(1 hr 20 min hands-on)
Price
Special offer
Online exclusive
Online offer:
Cat # ERA57RB
Invitrogen
Rat TNF alpha Uncoated ELISA Kit
Invitrogen
Rat TNF alpha Uncoated ELISA Kit

7 References

Sensitivity 16 pg/mL
Assay Range 16-2,000 pg/mL
Sample Volume
Plasma
100 µL
Serum
100 µL
Supernatant
100 µL
Time To Result
24 hr 30 min
(1 hr 45 min hands-on)
Price
Special offer
Online exclusive
Online offer:
Cat # 88-7340-88
Invitrogen
Rat TNF alpha Uncoated ELISA Kit with Plates
Invitrogen
Rat TNF alpha Uncoated ELISA Kit with Plates

9 References

Sensitivity 16 pg/mL
Assay Range 16-2,000 pg/mL
Sample Volume
Plasma
100 µL
Serum
100 µL
Supernatant
100 µL
Time To Result
24 hr 30 min
(1 hr 45 min hands-on)
Price
Special offer
Online exclusive
Online offer:
Cat # 88-7340-22
Invitrogen
Rat TNF alpha ELISA Development Kit (ABTS), PeproTech®
Invitrogen
Rat TNF alpha ELISA Development Kit (ABTS), PeproTech®
Sensitivity Lot-specific
Assay Range Lot-specific
Sample Volume
Time To Result
24 hr 30 min
(1 hr 45 min hands-on)
Price
Special offer
Online exclusive
Online offer:
Cat # 900-K73K

10 x 96 Tests

Invitrogen
Rat TNF alpha ELISA Development Kit (TMB), PeproTech®
Invitrogen
Rat TNF alpha ELISA Development Kit (TMB), PeproTech®
Sensitivity Lot-specific
Assay Range Lot-specific
Sample Volume
Time To Result
24 hr 30 min
(1 hr 45 min hands-on)
Price
Special offer
Online exclusive
Online offer:
Cat # 900-T73K

10 x 96 Tests

Invitrogen
Monkey TNF alpha (Total) ELISA Kit
Invitrogen
Monkey TNF alpha (Total) ELISA Kit
Sensitivity 6.0 U/mL
Assay Range 28.1-1,800 U/mL
Sample Volume
Serum
50 µL
Supernatant
50 µL
Time To Result
3 hr 10 min
(1 hr 20 min hands-on)
Price
Special offer
Online exclusive
Online offer:
Cat # BMS654
Invitrogen
Porcine TNF alpha ELISA Kit
Invitrogen
Porcine TNF alpha ELISA Kit

22 References

Sensitivity <3 pg/mL
Assay Range 23.4-1,500 pg/mL
Sample Volume
Plasma
100 µL
Serum
100 µL
Supernatant
100 µL
Time To Result
5 hr
(1 hr 20 min hands-on)
Price
Special offer
Online exclusive
Online offer:
Cat # KSC3011
Invitrogen
Canine TNF alpha ELISA Kit
Invitrogen
Canine TNF alpha ELISA Kit
Sensitivity 2 pg/mL
Assay Range 2.87-700 pg/mL
Sample Volume
Plasma
50 µL
Serum
50 µL
Supernatant
100 µL
Time To Result
4 hr 45 min
(1 hr 20 min hands-on)
Price
Special offer
Online exclusive
Online offer:
Cat # ECTNF
Invitrogen
Porcine TNF-alpha ELISA Kit
Invitrogen
Porcine TNF-alpha ELISA Kit
Sensitivity 20 pg/mL
Assay Range 20-10 ng/mL
Sample Volume
Plasma
50 µL
Supernatant
50 µL
Serum
50 µL
Time To Result
4 hr 45 min
(1 hr 20 min hands-on)
Price
Special offer
Online exclusive
Online offer:
Cat # ES24RB
Invitrogen
Rabbit TNF alpha ELISA Kit
Invitrogen
Rabbit TNF alpha ELISA Kit
Sensitivity 9.38 pg/mL
Assay Range 15.63-1,000 pg/mL
Sample Volume
Serum
100 µL
Plasma
100 µL
Time To Result
3 hr 30 min
(1 hr 20 min hands-on)
Price
Special offer
Online exclusive
Online offer:
Cat # EEL131

96 Tests

Invitrogen
Bovine TNF alpha ELISA Kit
Invitrogen
Bovine TNF alpha ELISA Kit
Sensitivity 0.1 ng/mL
Assay Range 0.123-30 ng/mL
Sample Volume
Plasma
50 µL
Serum
50 µL
Supernatant
100 µL
Time To Result
4 hr 45 min
(1 hr 20 min hands-on)
Price
Special offer
Online exclusive
Online offer:
Cat # EBTNF
Invitrogen
Equine TNF alpha Uncoated ELISA Kit
Invitrogen
Equine TNF alpha Uncoated ELISA Kit

3 References

Sensitivity <15.6 pg/mL
Assay Range 15.6-1,000 pg/mL
Sample Volume
Supernatant
100 µL
Time To Result
24 hr
(2 hr 50 min hands-on)
Price
Special offer
Online exclusive
Online offer:
Cat # ESS0017

5 x 96 Tests

Invitrogen
Porcine TNF alpha Matched Antibody Pair
Invitrogen
Porcine TNF alpha Matched Antibody Pair

3 References

Sensitivity <62.5 pg/mL
Assay Range 62.5-4,000 pg/mL
Sample Volume
Plasma
100 µL
Serum
100 µL
Supernatant
100 µL
Time To Result
24 hr
(1 hr 30 min hands-on)
Price
Special offer
Online exclusive
Online offer:
Cat # CSC1753

10 x 96 Tests

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